구강암에서 림프관형성 인자의 발현에 관한 면역조직화학적 연구
Immunohistochemical Study on Expression of Lymphangiogenic Factors in Oral Cancer
박영욱, 권광준, 이종원,
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박영욱 ( Park Young-Wook ) - 강릉원주대학교 치과대학 구강악안면외과학교실
권광준 ( Kwon Kwang-Jun ) - 강릉원주대학교 치과대학 구강악안면외과학교실
이종원 ( Lee Jong-Won ) - 강릉원주대학교 치과대학 구강악안면외과학교실
KMID : 0360120100320010001
Abstract
Background and Purpose: Vascular endothelial growth factor (VEGF)-C and VEGF receptor (VEGFR)-3 are involved in tumor lymphangiogenesis. Oral mucosal squamous cell carcinoma (OMSCC) preferentially metastasizes to cervical lymph nodes, so we investigated the expression and distribution of VEGFR-3 signaling proteins in OMSCC.
Materials and Methods: Tissue samples of 18 OMSCC, 10 oral mucosal leukoplakia, and 3 normal oral mucosa were evaluated for expression of VEGF-C, VEGF-D, and VEGFR-3 by immunohistochemical staining. The presence of lymphatic vessels was determined using D2-40 staining, by which we also measured lymphatic vessel density (LVD).
Results: 72% (13/18) and 56% (10/18) of tissue samples showed VEGF-C and VEGF-D immunopositivity in tumor cells and tumor-associated endothelial cells. VEGFR-3 was also expressed in most of OMSCC, which was up-regulated when compared with normal mucosa or with leukoplakia. Furthermore, LVD was higher in OMSCC than in leukoplakia.
Conclusion: Taken together, our results suggest that autocrine activation of lymphatic endothelial cell via VEGFR-3 by VEGF-C and/or VEGF-D could be involved in progression of OMSCC. Therefore, VEGF-C/VEGFR-3 signaling pathway can be a molecular target for anti-metastatic therapy in OMSCC.
키워드
Oral mucosal squamous cell carcinoma;Lymphangiogenesis;Lymphatic vessel density
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